Note
Click here to download the full example code
Vascular Reconstruction¶
This example shows how to perform vascular reconstruction based on MP2RAGE data by performing the following steps:
- Downloading three open MP2RAGE datasets using
nighres.data.download_7T_TRT()
[1]
- Remove the skull and create a brain mask using
nighres.brain.mp2rage_skullstripping()
- Vasculature reconstruction using
nighres.filtering.multiscale_vessel_filter()
[2]
Important note: this example extracts arteries as bright vessels in a T1-weighted 7T scan, instead of the veins extracted from QSM in [2], because these data sets could not be made openly available. Processing of QSM images would however follow the same pipeline
Import and download¶
First we import nighres
and the os
module to set the output directory
Make sure to run this file in a directory you have write access to, or
change the out_dir
variable below.
import nighres
import os
import nibabel as nb
import numpy as np
import matplotlib.pyplot as plt
in_dir = os.path.join(os.getcwd(), 'nighres_examples/data_sets')
out_dir = os.path.join(os.getcwd(), 'nighres_examples/vascular_reconstruction')
We also try to import Nilearn plotting functions. If Nilearn is not installed, plotting will be skipped.
skip_plots = False
try:
from nilearn import plotting
except ImportError:
skip_plots = True
print('Nilearn could not be imported, plotting will be skipped')
Now we download an example MP2RAGE dataset. It is the structural scan of the first subject, first session of the 7T Test-Retest dataset published by Gorgolewski et al (2015) [1].
dataset = nighres.data.download_7T_TRT(in_dir, subject_id='sub001_sess1')
Skull stripping¶
First we perform skull stripping. Only the second inversion image is required
to calculate the brain mask. But if we input the T1map and T1w image as well,
they will be masked for us. We also save the outputs in the out_dir
specified above and use a subject ID as the base file_name.
skullstripping_results = nighres.brain.mp2rage_skullstripping(
second_inversion=dataset['inv2'],
t1_weighted=dataset['t1w'],
t1_map=dataset['t1map'],
save_data=True,
file_name='sub001_sess1',
output_dir=out_dir)
Tip
in Nighres functions that have several outputs return a
dictionary storing the different outputs. You can find the keys in the
docstring by typing nighres.brain.mp2rage_skullstripping?
or list
them with skullstripping_results.keys()
To check if the skull stripping worked well we plot the brain mask on top of
the original image. You can also open the images stored in out_dir
in
your favourite interactive viewer and scroll through the volume.
Like Nilearn, we use Nibabel SpatialImage objects to pass data internally. Therefore, we can directly plot the outputs using Nilearn plotting functions .
if not skip_plots:
plotting.plot_roi(skullstripping_results['brain_mask'], dataset['t1map'],
annotate=False, black_bg=False, draw_cross=False,
cmap='autumn')
Vessel reconstruction¶
Next, we use the vessel filter to estimate the vasculature from the QSM data
vessel_result = nighres.filtering.multiscale_vessel_filter(
input_image=skullstripping_results['t1w_masked'],
scales=2,
save_data=True, file_name="sub001_sess1",
output_dir=out_dir)
Now we look at the topology-constrained segmentation MGDM created
if not skip_plots:
plotting.plot_img(vessel_result['pv'],
vmin=0, vmax=1, cmap='cubehelix', colorbar=True,
annotate=False, draw_cross=False)
plotting.plot_img(vessel_result['diameter'],
vmin=0, vmax=4, cmap='cubehelix', colorbar=True,
annotate=False, draw_cross=False)
If the example is not run in a jupyter notebook, render the plots:
if not skip_plots:
plotting.show()
Additional visualization: compute maximum intensity projections
data = nighres.io.load_volume(vessel_result['pv']).get_fdata()
fig, ax = plt.subplots(1, 3, figsize=(28,5))
ax[0].imshow(np.rot90(np.max(data[100:130,:,:], axis=0)), cmap = 'gray')
ax[1].imshow(np.rot90(np.max(data[:,170:200,:], axis=1)), cmap = 'gray')
ax[2].imshow(np.rot90(np.max(data[:,:,170:200], axis=2)), cmap = 'gray')
for i in range(3):
ax[i].set_xticks([])
ax[i].set_yticks([])
fig.tight_layout()
#fig.savefig('segmentation.png')
plt.show()
References¶
[1] | (1, 2) Gorgolewski et al (2015). A high resolution 7-Tesla resting-state fMRI test-retest dataset with cognitive and physiological measures. DOI: 10.1038/sdata.2014.54 |
[2] | (1, 2) Huck et al. (2019) High resolution atlas of the venous brain vasculature from 7 T quantitative susceptibility maps. DOI: 10.1007/s00429-019-01919-4 |
Total running time of the script: ( 0 minutes 0.000 seconds)